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Hooykaas, P. J. , 1982: Tumors induced by different strains of Agrobacterium tumefaciens. , Schell, J.

Garfinkel et al. , 1983 a). Varying results have also been obtained with mutants in gene 5. , 1982). , 1983 a) induce apparently normal tumors. These genes may have a minor role in tumor formation or intervene in the synthesis of as yet unidentified opines. There are some important conclusions to draw from the analysis of all the T-DNA mutants: no single function is absolutely necessary for tumor induction, only deletions removing several genes (e. g. , 1983 b). No tumor is formed in this case but the wound callus can be tested for opines and is usually positive (see also IILF.

Gheysen, P. Dhaese, M. Van Montagu, and J. , 1980; Doerfler, 1982). , 1981). The T-DNA is similar to transposons in its ability to integrate into different nonhomologous places in the plant genome. , 1983). , 1982). F. Crossing the Cellular and Genetic Barriers There are several models consistent with the data presented here that can account for the introduction of T-DNA into the plant cell. One possibility is that the T-region is excised from the Ti plasmid within the bacteria, and subsequently transferred alone to the plant cells.

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